Denne preprinten landet her i går. Og gjett om de bruker SAN 🤓 fra «articzymes»🥴 liten skrivefeil der… 🙃

AAV producing 293T cell pellets were extracted using a lysis buffer containing salt active nuclease (articzymes) in 40 mM Tris, 500 mM NaCl and 2 mM MgCl2 pH 8 (referred to as SAN buffer).

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We thank O. Mazor and P. Gorelik (HMS Research Instrumentation Core) for help with design and construction of the GCaMP imaging setup and stimulation devices, and Ginty lab and Sharma lab members for discussions and comments on the manuscript. We thank Caiying Guo and the Gene Targeting and Transgenics Facility at Janelia Research Campus for generating mouse lines. This work was supported by a Quan Predoctoral Fellowship (L.Q.), NIH grants NS097344 (D.D.G.), AT011447 (D.D.G.), 1DP2NS127278 (N.S.), The Klingenstein-Simons Foundation (N.S.), The Whitehall Foundation (N.S.), The Bertarelli Foundation (D.D.G.), The Hock E. Tan and Lisa Yang Center for Autism Research (D.D.G.), and the Lefler Center for Neurodegenerative Disorders (D.D.G.). D.D.G. is an HHMI investigator. This article is subject to HHMI’s Open Access to Publications policy. HHMI lab heads have previously granted a nonexclusive CC BY 4.0 license to the public and a sublicensable license to HHMI in their research articles. Pursuant to those licenses, the author-accepted manuscript of this article can be made freely available under a CC BY 4.0 license immediately upon publication.

https://www.biorxiv.org/content/10.1101/2023.04.22.537932v1.full.pdf